If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
To our knowledge, customised protocols are not required for this product. Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP(V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours 図2 IP Result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug. Chem. The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). Southern,J.A.,Young,D.F.,Heaney, F.,Baumgartner,W.K.,Randall,R.E.,1991.Identiﬁcation of an epitope on the P and V proteins of simian virus 5 that distinguishes between two isolates with different biological characteristics.
Specializing in small molecule inhibitors! Please try the standard protocols listed below and let us know how you get on. Order by school mailbox. Evaluation sample solution : ship with blue ice.
Compact insect cell vector with a Zeocin™ resistance marker, for constitutive expression of proteins with an optional C-terminal V5-6xHis tag. Blocking - Blocking peptide for Anti-V5 tag antibody (Agarose) (ab1229), Anti-V5 tag antibody (FITC) (ab1274), Anti-V5 tag antibody (HRP) (ab1325), Anti-V5 tag antibody (ab9113), Anti-V5 tag antibody (ab9116), Anti-V5 tag antibody (ab9137), Anti-V5 tag antibody (ab15828).
pIZ V5-His.dna Map and Sequence File: Download Open Home » Resources » Plasmid Files » Mammalian Expression Vectors » pcDNA3.1 V5-His C. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer. L. Huang, Y. Lu et al. Like c-myc and HA tags, the V5 tag is a small epitope tag (IPNPLLGLD), which can be placed by molecular cloning at the N- or C-terminus of a protein of choice. 161 (2011) 115–1234. © 1998-2020 Abcam plc. J. Biol. Please note: All products are "FOR RESEARCH USE ONLY. Shipped at 4°C.